Originally Posted by
kecked
If the requirement is as fast as possible and a coherent light source I again go back to several non scanned solution.
You first need to tell us the wavelength of interest. If it is Blue you have up to 9W of light in one diode in as small a beam as you like which will incinerate the plant. This means you need to define an upward bound of the light input and afford the opportunity to spread the beam using optics and skip scanning entirely. There is an upward boundary for photosynthesis limited by the ability of the plant to supply the required ingredients and remove the waste products and even more important is heat. Even if the plant can keep up, the rate of reaction increases rapidly with rising heat. So you are introducing variables you are not controlling. That means that your results will be garbage.
You MUST insure that your keep the temperature at the same as your control. You can't do that just by measuring the leaf temperature because as the beam hot spot moves you are very rapidly heating and then cooling much faster than you can see with a thermometer or maybe even a photometer.
It seems to me the first thing you need to do is determine the flux of photons you want as a control in say lumens/cm2 and then assure you have the same flux on the test area. It will be extremely important to diffuse the coherent light and NOT to scan a concentrated beam on the leaf by spreading the beam to produce a uniform flux like the control.
Now once you do that then you can start asking questions like can I essentially q switch photosynthesis by rapidly scanning an area in such a way the leaf has time to recover from the excessive radiant input, thermal load, and waste removal issues.
It seems to me you could make a much more robust light source by using a xenon lamp and a monochromator. You then need only expand a single axis with a cylinder lens to make the square shape you desire.
I must be missing something you intend to do because this experiment does not make a lot of sense. Are you planning to take samples of tissue and look at the chromophores and chloplasts to see morphology changes or do analysis for CO2 uptake changes? Maybe do electrode implants to watch redux changes?
Last, and I think this is most important, how in the world are you thinking you can see growth changes in a plant on such a small scale of the entrie plant and isolate the background input from other sources of light while not killing the plant in the process. You surely will not see changes immediately except maybe smoke from 9W scanned on a leaf. You have to keep the plant alive. Ok one more point, no two plants are exactly alike so I suggest you get a clone which is available.
I'm not trying to piss you of or make you feel bad. I'm just saying that if you are going to take the time to do this you should know that there are a lot smarter people than me that will peer review you into a closet.