
Originally Posted by
femtoman
Three-photon microscopy (3PEF) is a high-resolution fluorescence microscopy based on nonlinear excitation effect. Different from two photon excitation microscopy, it uses three exciting photons. It typically uses 1300 nm or longer wavelength lasers to excite the fluorescent dyes with three simultaneously absorbed photons. The fluorescent dyes then emit one photon whose energy is (slightly smaller than) three times the energy of each incident photon.
You can find on the net large quantity of publication of the tree photon application since 1995 !